Fig. 1

Identification of a novel lncRNA-HGBC whose expression levels were associated with the progression of GBC. a Box and whisker plots of the relative expression of lncRNA-HGBC in 43 paired human GBC tissues and non-tumour tissues (NT). b Kaplan-Meier survival curves of 43 gallbladder cancer patients with the high- and low-lncRNA-HGBC expression. The median expression level of lncRNA-HGBC was used as the cutoff. c 5′ and 3′ rapid amplification of cDNA ends (RACE) assays in NOZ cells to detect the whole sequence of lncRNA-HGBC. Left; a gel electrophoresis image of PCR products from the 5′-RACE and 3′-RACE assays. Right; sequencing of PCR products indicated the boundary between the universal anchor primer and lncRNA-HGBC sequences. d Northern blot analysis to confirm the length and expression of the lncRNA- HGBC in GBC tissues and cells. β-Actin was used as a loading control. e Fluorescence in Situ Hybridization (FISH) assay (green) used to examine the expression and location of lncRNA-HGBC in SGC-996 and NOZ cells. Scale bars, 50 μm. f In vitro translation assay using luciferase (Luc) as a positive control and lncRNA-HGBC templates as described in the Methods