Fig. 2

circERBB2 promotes growth of GBC cells in vitro. a Deletion of 3′ flanking Alu repeat was achieved with two sgRNAs and verified by PCR using primer pair targeting flanking of sgRNA targeted region. b qPCR showed that circERBB2, but not ERBB2 mRNA, was knock-down by deletion of 3′ flanking Alu repeat (n = 3). c CCK8 assay showed that knock-down of circERBB2 by CRISPR-cas9 impaired proliferation of GBC cells (n = 3). d Knock-down of circERBB2 by CRISPR-cas9 impaired clone formation ability of GBC cells. e OE of circERBB2 promoted proliferation of GBC cells (n = 3). f OE of circERBB2 promoted clone formation of GBC cells. Quantitative data from three independent experiments was presented as mean ± SD (error bars). P-values were determined by paired, two-tailed two sample t-test. *: p < 0.05; **: p < 0.01