Fig. 9

BCL2 is a target of miR-6785-5p and is suppressed by MNX1-AS1 deletion. a qRT-PCR assays were used to detect miR-6785-5p in GC cells transfected with miR-6785-5p mimic or miR-6785-5p inhibitor compared with each control group. b MTT assays were performed to investigate the effects of altered miR-6785-5p expression on the cell viability in GC. c SGC7901 and MGC803 cells transfected with miR-NC or miR-6785-5p were stained and analyzed by cell apoptosis assays. d The expression level of BCL2 was remarkably downregulated in GC cells with MNX1-AS1 knockdown. e qRT-PCR assays were used to determine the effects of miR-6785-5p dysregulation on BCL2 expression in SGC7901 and MGC803 cells. f The prediction result of miR-6785-5p putative targeting site in the WT and Mut 3′ UTR of BCL2. MiR-6785-5p could bind to the predicted site in the 3’UTR of BCL2 mRNA. g Western blot assays revealed that miR-6785-5p overexpression can impair the BCL2 upregulation mediated by MNX1-AS1 overexpression, while miR-6785-5p suppression can reduce the BCL2 downregulation induced by MNX1-AS1 knockdown