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Fig. 6 | Molecular Cancer

Fig. 6

From: LncRNA LINRIS stabilizes IGF2BP2 and promotes the aerobic glycolysis in colorectal cancer

Fig. 6

LINRIS could be inhibited by GATA3 in CRC. a Predicted GATA3 DNA-binding sequences are present in the human LINRIS promoter region. b GATA3 expression in several CRC microarray datasets from Oncomine database. c Kaplan-Meier analysis of the OS curves for CRC patients with different GATA3 expression from TCGA database (log-rank test). d LINRIS mRNA levels were upregulated with knockdown of GATA3 in CRC cells. The data are shown as the mean ± SD; n = 3 independent experiments, two-tailed Student’s t-test, *P < 0.05, **P < 0.01. e ChIP-PCR of LINRIS in CRC cells. Error bars, SD of three independent experiments. *P < 0.05 or **P < 0.01. f Relative LINRIS luciferase promoter activity in the indicated cells with GATA3 overexpression (OE-GATA3). Error bars, SD of three independent experiments. *P < 0.05 or **P < 0.01 versus the control. g Relative LINRIS luciferase promoter activity in the indicated cells with GATA3 knockdown. Error bars, SD of three independent experiments. *P < 0.05 or **P < 0.01 versus the control. h Relative LINRIS luciferase promoter activity in the indicated cells with or without the mutation of GATA3 binding sequences. Error bars, SD of three independent experiments. *P < 0.05 or **P < 0.01 versus the control. i Correlations between the LINRIS levels and the mRNA levels of GATA3 in CRC (n = 76). RNA levels were determined by qPCR relative to the levels of GAPDH. The r values and P values are from Pearson’s correlation analysis. j Representative IHC images of GATA3 in tumor tissues from patients with CRC (n = 220) with low or high levels of LINRIS. Scale bar, 50 μm. k Percentages of specimens showing different levels of GATA3 in the low or high LINRIS expression groups (n = 220, Chi-square test, **P < 0.01)

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