Fig. 1

LCAT1 is upregulated in lung cancer tissues and is associated with poor prognosis. a Schematic representation of LCAT1 exons and transcripts and its loci on human chromosome 2q31.1. b The protein coding potential of LCAT1 evaluated by the Coding Potential Assessment Tool (CPAT) and Coding Potential Calculator 2 (CPC2). A smaller CAPT/CPC2 score indicates low probability of protein coding potential. c Relative expression of LCAT1 in lung tumor tissues and adjacent normal tissues in TCGA cohort. The expression of LCAT1 was quantified by fragments per kilobase of transcript per million reads mapped (FPKM) in the RNA sequencing data. d, e LCAT1 expression validated by qRT-PCR in an independent cohort of 25 pairs of lung cancer tissues and matched adjacent normal tissues. LCAT1 expression was normalized to the expression of actin. f, g, h, i Difference in the expression of LCAT1 between lung adenocarcinoma patients with somatic mutations in EGFR (f), ALK (g), ROS (h) or NRAS (i) and those without such mutations. j, k Kaplan-Meier curves of overall survival (j) and disease-specific survival (k) based on LCAT1 expression levels. Error bars indicate standard error of mean (SEM). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001