Fig. 2

LCAT1 promotes lung cancer cell proliferation in vitro and in vivo. a qRT-PCR analysis of LCAT1 expression in lung cancer cells. b Relative expression of LCAT1 in lung cancer cells transfected with LCAT1 siRNA (si-LCAT1 2# or si-LCAT1 4#) and scrambled siRNA. c Growth curve of lung cancer cells transfected with LCAT1 siRNA or scrambled siRNA by CCK-8 assays. d, e Proliferation of lung cancer cells transfected with LCAT1 siRNA or scrambled siRNA as determined by colony formation assay (d) and EdU staining assay (e). f, g Tumor volume and weight of mouse xenografts subcutaneously injected with A549 cells with stable LCAT1 knockdown. The tumor growth curve was measured every 3 days. Nude mice were euthanized 3 weeks following treatment and the tumor nodules were collected. All in vitro experiments were performed in triplicate and one of representative results was presented. Error bars indicate standard error of mean (SEM). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. The below is same for other figures