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Fig. 4 | Molecular Cancer

Fig. 4

From: Long noncoding RNA LCAT1 functions as a ceRNA to regulate RAC1 function by sponging miR-4715-5p in lung cancer

Fig. 4

LCAT1 interacts with miR-4715-5p in lung cancer cells. a, b LCAT1 localization was predicted using lncRNA subcellular localization predictor, lncLocator (a) and subcellular fractionation (b). c RIP experiments were performed in A549 cells and the coprecipitated RNA was used to quantify LCAT1 expression using qRT-PCR. d, e Potential miRNA binding to LCAT1. miRNA-LCAT1 interaction was predicted by miRanda and RegRNA2, of which 17 were overlapped. Several common miRNAs were further validated using a luciferase reporter assay. f Luciferase reporter assay shows that miR-4715-5p binds to LCAT1. The luciferase reporter plasmid containing wildtype (Wt) or mutant (Mut) LCAT1 was co-transfected into A549 cells with miR-4715-5p mimics or miR-NC mimics. g miR-4715-5p expression by qPCR in lung cancer cells transfected with control siRNA or si-LCAT1. U6 was used as an internal control gene for the quantification of miRNA expression

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