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Fig. 2 | Molecular Cancer

Fig. 2

From: N6-methyladenosine induced miR-143-3p promotes the brain metastasis of lung cancer via regulation of VASH1

Fig. 2

miR-143-3p triggers EMT, invasion of BBB model, and angiogenesis of lung cancer cells. a The quantitative results of wound healing assay of A549 cells transfected with miRNAs for 48 h. b The relative expression of miR-143-3p in lung cancer cell A549, H1975, H292, H1650 and H1299 cells, human bronchial epithelial cells (HBEC), HBMEC, HUVEC, and pulmonary artery endothelial cells (PAEC) was checked by qRT-PCR. c Cells were transfected with scramble RNA (Con) or miRNA-143 for 24 h, the cell invasion was measured by use of transwell assay and quantitatively analyzed. d A549 cells were transfected with control or miR-143-3p for 48 h, the expression of cell motility markers was measured by western blot analysis (left) and quantitatively analyzed (right). e The in vitro models of BBB. HBMECs grown on a Transwell membrane. f A549 or H1975 cells transfected with or without miR-143-3p were allowed to invade the in vitro BBB model for 48 h. The invaded cells were measured (left) and quantitatively analyzed (right). g Tube formation was evaluated in HUVECs cultured for 12 h in medium collected from A549 or H1975 cells transfected with or without miR-143-3p. The tube formation was recorded (left) and quantitatively analyzed (right). h After transfected with or without miR-143-3p for 24 h, the expression of VEGFA in lung cancer cells was measured by western blot analysis (left) and quantitatively analyzed (right). i H1299 cells transfected with or without miR-143-3p inhibitor were allowed to invade the in vitro BBB model for 48 h. The invaded cells were measured (left) and quantitatively analyzed (right). j Tube formation was evaluated in HUVECs cultured for 12 h in medium collected from H1299 cells transfected with or without miR-143-3p inhibitor. The tube formation was recorded (left) and quantitatively analyzed (right). k After transfected with or without miR-143-3p inhibitor for 24 h, the expression of VEGFA in H1299 cancer cells was measured by western blot analysis (left) and quantitatively analyzed (right). Data are presented as the mean ± SD from three independent experiments. *p < 0.05, ** p < 0.01 compared with control. Scale bar = 100 μm

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