Fig. 3

VASH1 mediates miR-143-3p induced cell dissemination and angiogenesis. a & b A549 (A) or H1975 (b) cells were transfected with control or miR-143-3p for 24 h, the mRNA expression of potential targets was measured by qRT-PCR. c Lung cancer cells were transfected with control or miR-143-3p for 24 h, the expression of VASH1 was measured by western blot analysis (left) and quantitatively analyzed (right). d The relative expression of miR-143-3p and mRNA of VASH1 was negative correlated in measured lung cancer and HBEC cells. e Schematic representation of three positions of miR-143-3p binding with the 3’UTR of VASH1 mRNA. f A549 cells were co-transfected with control, miR-143-3p, pmiR-GLO-WT, and pmiR-GLO-Mut-a/b/c for 24 h, the value of firefly luciferase (F-luc) was normalized to that of the Renilla luciferase (R-luc). g-i A549 cells were co-transfected with control, miR-143-3p, vector, or VASH1 construct for 48 h, (g) the expression of FN and E-Cad was measured by measured by western blot analysis (left) and quantitatively analyzed (right); h The invasion of BBB model for 48 h was measured (left) and quantitatively analyzed (right); i The tube formation was evaluated in HUVECs cultured for 12 h (left) and quantitatively analyzed (right). Data are presented as the mean ± SD from three independent experiments. *p < 0.05, ** p < 0.01 compared with control, NS, no significant. Scale bar = 100 μm