Fig. 5

VASH1 mediates miR-143-3p induced depolymerization of microtube. a A549 cells were transfected with control, miR-143-3p, vector or VASH1 construct for 24 h, the expression of deY-Tub and tubulin was measured by western blot analysis (left) and quantitatively analyzed (right). b The expression and localization of α-tubulin (red) and VASH1 (green) in control and miR-143-3p stable A549 cells were checked by confocal. c The expression and localization of α-tubulin (red) and deY-Tub (green) in control and miR-143-3p stable A549 cells were checked by confocal. d The control and miR-143-3p stable A549 cells were treated with or without PTX (2 μM) for 2 h, the expression of deY-Tub was measured by western blot analysis (left) and quantitatively analyzed (right). e The control and miR-143-3p stable A549 cells were treated with or without PTX (2 μM) for 2 h, the invasion of BBB model for 24 h was recorded (left) and quantitatively analyzed (right). f Immunofluorescence staining of microtubule re-growth of control and miR-143-3p stable A549 cells transfected with or without VASH1 construct after treatment with nocodazole (50 μM) for 10 min, followed by a washout and incubation in fresh culture medium at 37 °C for 10, 30, or 120 min. g Control and miR-143-3p stable A549 cells transfected with or without VASH1 construct after treatment with nocodazole (50 μM) for 10 min, followed by a washout and incubation in fresh culture medium at 37 °C for 10, 30, or 120 min. The levels of deY-tub and total tubulin were measured by western blot analysis (left) and quantitatively analyzed (right). h A549 cells were transfected with control, miR-143-3p, vector or VASH1-C169A- construct for 24 h, the expression of deY-Tub and tubulin was measured by western blot analysis (left) and quantitatively analyzed (right). i The control and miR-143-3p stable A549 cells were transfected with vector or VASH1-C169A construct for 24 h, the invasion of in vitro BBB model was recorded (left) and quantitatively analyzed (right). j A549 cells were transfected with vector control or VASH1-C169A construct for 24 h, the protein expression was recorded (left) and quantitatively analyzed (right). Data are presented as the mean ± SD from three independent experiments. *p < 0.05, ** p < 0.01 compared with control. Red scale bar = 100 μm. White scale bar = 10 μm