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Fig. 6 | Molecular Cancer

Fig. 6

From: N6-methyladenosine induced miR-143-3p promotes the brain metastasis of lung cancer via regulation of VASH1

Fig. 6

The m6A regulates the expression of miR-143-3p in lung cancer cells. a Cells were transfected with si-NC or si-Mettl3 for 24 h, the expression of miR-143-3p was checked by qRT-PCR. b Cells were transfected with vector control or Mettl3 constructs for 24 h, the expression of miR-143-3p was checked by qRT-PCR. c A549 cells were transfected with sh-Control or sh-Mettl3 for 24 h, the expression of precursor miR-143-3p was measured by qRT-PCR. d A549 cells were transfected with (sh-Control) or sh-Mettl3 for 24 h, the expression of precursor miR-143-3p in cytoplasm and nucleus was measured by qRT-PCR. e & f A549 cells were transfected with sh-Control or sh-Mettl3 for 24 h and further treated with Act-D for the indicated times, and mature and precursor miR-143-3p expression was measured by qRT-PCR. g m6A RIP-qPCR analysis of precursor and mature miR-143-3p in A549 cells. h A549 cells were transfected with si-NC or si-Dicer for 24 h, the expression of Dicer was measured by western blot analysis. i A549 cells were transfected with vector control, Mettl3 construct, si-NC or si-Dicer alone or together for 24 h, the expression of mature miR-143-3p was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 compared with control

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