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Fig. 7 | Molecular Cancer

Fig. 7

From: N6-methyladenosine induced miR-143-3p promotes the brain metastasis of lung cancer via regulation of VASH1

Fig. 7

The miR-143-3p/VASH axis and in vivo BM of lung cancer. a The expression of VASH1, deY-tubulin, CD31, FN, and Vim in control and miR-143-3p groups were measured by IHC (left) and quantitatively analyzed (right). b & c The control and miR-143-3p A549 cells were injected into the nude mice by tail vein injection. Representative images of metastatic lung tumors and the H&E staining results were shown (left), and the number of lung tumors were quantitatively analyzed (right). d Metastasis was detected by histology; BM (100 X) and BM (200 X). e Percentage of BM in all measured groups. f Expression of VASH1 in NSCLC (cancer) and adjacent normal tissues from Oncomine dataset (Selamat). g VASH1 expression in lung cancers of T1 (n = 145), T2 (n = 182), and T3 (n = 35) stages from TCGA database. h Kaplan-Meier survival curves of OS based on VASH1 mRNA expression in lung cancer patients. The log-rank test was used to compare differences between two groups. i The IHC scores of VASH1 in BM+ (n = 34) or BM- (n = 32) lung cancer patients. j The IHC scores of VASH1 in miR-143-3p+ (n = 38) or miR-143-3p- (n = 28) lung cancer patients. (K) Kaplan-Meier survival curves of OS of miR-143-3pVASH1high and miR-143-3p+VASH1low group of lung cancer patients. The log-rank test was used to compare differences between two groups. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 compared with control. Scale bar = 100 μm

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