Fig. 4
CircFGFR1 regulates the miR-381-3p/CXCR4 pathway in the NSCLC cells. a Putative binding sites of miR-381-3p with respect to CXCR4 were predicated via StarBase v3.0. b The luciferase activity of pLG3-CXCR4 in the HEK-293 T cells cotransfected with miR-381-3p. c-f The levels of CXCR4 mRNA and protein were measured by RT-qPCR or western blotting in the NSCLC cells transfected with circFGFR1, shcircFGFR1, or the control. g Representative NSCLC cases were analyzed by IHC staining for CXCR4. h The CXCR4 levels in 210 pairs of NSCLC and matched nontumor tissues, shown as log2 (tumor/nontumor). i A positive correlation between circFGFR1 and CXCR4 expression was observed in the NSCLC tissues (R2 = 0.3169; P < 0.0001). j A negative correlation between circFGFR1 and miR-381-3p expression was observed in the NSCLC tissues (R2 = 0.3520; P < 0.0001). The data are presented as the mean ± SD. NS: not significant, *P < 0.05, **P < 0.01