Fig. 3

Flow cytometry analysis of BAY-1082439 in attenuating the anti-apoptosis ability of the MDR cell lines KB-C2 (a) and H460/MX80 (b) with the presence of colchicine (Col) and mitoxantrone (Mit) during 48 h of culture. The rate of cell necrosis (Nec) and apoptosis (Apo) with and without BAY-1082439 (BAY) was compared. The drug-sensitive cells KB-3-1 and H460 showing no apparent change of necrosis and apoptosis after treatment with BAY-1082439, were set as control for KB-C2 and H460/MX80, respectively. The dosage of BAY-1082439 was 10 μM. Colchicine was applied at 2 μM to KB-C2 and 0.02 μM to KB-3-1. Mitoxantrone was applied at 10 μM to H460/MX80 and 0.1 μM to H460. BAY-1082439 was applied at 10 μM to all the tested cells. Annexin V-FITC binding to phosphatidylserine (PS) that translocates to the external leaflet of the apoptotic cells was determined via FITC-A detection mode (Ex: 488 nm, Em: 525 nm). Propidium iodide (PI) that binds nucleotides of late-apoptotic or necrotic cells was determined via violet 610-A detection mode (Ex: 450 nm; Em: 610 nm) to avoid excitation of FITC. The four cell lines in healthy status were labelled with H1-H4