Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: The PI3K subunits, P110α and P110β are potential targets for overcoming P-gp and BCRP-mediated MDR in cancer

Fig. 6

Immunoprecipitation analysis showing the effect of inhibition of P110α and P110β on the expression level of AKT and some pivotal proteins regulated by AKT. Western blot for the analysis of Pan-AKT (a) and series of its downstream factors (b) was performed after the KB-C2 and H460/MX80 MDR cells and the KB-3-1 and H460 drug sensitive parental cells were co-cultured with 10 μM of BAY-1082439 for 48 h. In addition to BAY-1082439, KB-3-1 and KB-C2 cells were also co-cultured with 0.1 and 1 μM of colchicine, and H460 and H460/MX80 cells were also co-cultured with 1 and 10 μM of mitoxantrone, respectively. Molecular for each protein: AKT: 60 kDa; GSK3β: 47 kDa; P53: 53 kDa; Phospho-FOXO3a (Ser253): 82–97 kDa; caspase-9: 46 kDa; Bcl-xL: 23.7 kDa

Back to article page

Molecular Cancer

ISSN: 1476-4598