Fig. 1
From: LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription
LINC02273 is a prognostic factor in breast cancer. a Schematic view of the chromosomal location of LINC02273 and the two isoforms confirmed by RACE-PCR. b Representative images from RACE PCR and Sanger sequencing. c Relative RNA expression of 1653 nt and 1252 nt isoform in MDA-MB-231 and BT549 cells analyzed by RT-qPCR. Normalized to GAPDH, n = 3, biological replicates. d LINC02273 localization in MDA-MB-231 revealed by FISH. Blue, DAPI; red, LINC02273 RNA. Scale bar, 5 μm. e Cytoplasmic and nuclear fractions were extracted from MDA-MB-231 and BT549 cells and LINC02273 expression was analyzed by RT-qPCR. n = 3, biological replicates. f Relative expression of LINC02273 in primary tumor and LN metastatic loci in a 55-paired-sample cohort (Wilcoxon matched-pairs signed rank test). Normalized to GAPDH. g RT-qPCR for LINC02273 expression in human breast cancer with different pathological nodal stages (pN, Kruskal-Wallis test). Normalized to GAPDH. h Relative expression of LINC02273 in primary tumors from patients suffered metastasis afterward or without metastasis (no event). Mann-Whitney test was used for the statistical analysis. Normalized to GAPDH. i Kaplan-Meier recurrence-free survival (RFS) analysis of LINC02273 expression in breast cancer patients (log rank test). Values are expressed as the median with interquartile range in (g)-(h), mean ± s.e.m. in (c). *P < 0.05 and ***P < 0.001