Fig. 2

LINC02273 promotes breast cancer metastasis. a and b Wound healing assay in MDA-MB-231 and BT549 cells. LINC02273 KO cells and WT cells were used. Representative photos and quantitative analysis were shown. Wound width was determined by IncuCyte. Scale bar, 300 μm. n = 3, biological replicates. c and d Transwell migration assay and invasion assay were performed in MDA-MB-231/LINC02273 KO and BT549 cells/LINC02273 KO and the corresponding control cells. Confluence of migrated cells were analyzed by ImageJ. Scale bar, 100 mm. n = 3, biological replicates. e Tumor volumes were measured in the WT and LINC02273 KO groups in the xenograft mouse model (unpaired Student’s t test was performed on the day 38, n = 8 of each group). f Representative images of the xenograft tumors isolated from three indicated groups. g Representative images of bioluminescence imaging (BLI) on lungs of mice 7 weeks after orthotopic injection of indicated cells. h Statistical analysis of lung area photon flux (n = 8, means ± s.e.m., Mann-Whitney test). i Representative images of H&E staining of lung metastasis. Two representative images were shown from each group. Scale bars: black, 2 mm; pink, 100 μm. a-e, means ± s.e.m. are shown, and P values were determined by independent sample t-tests. *P < 0.05, **P < 0.01, ***P < 0.001. WT, wild type. KO, knockout