Fig. 4
From: LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription
hnRNPL-LINC02273 promotes breast cancer progression. a Relative expression of hnRNPL in primary tumor and LN metastatic loci in a 49-patient cohort (Wilcoxon matched-pairs signed rank test). Normalized to GAPDH. b Correlation analysis between hnRNPL and LINC02273 in metastatic loci of LN. n = 52 samples (Spearman r). c Transwell migration and invasion assay after hnRNPL knockdown. d Transwell migration and invasion assay after hnRNPL ectopic overexpression in MDA-MB-231 and BT549 cells. Representative images and statistical analysis were shown. Scale bars: 100 μm. n = 3, biological replicates. e Transwell assay in WT and LINC02273 KO cells with hnRNPL overexpression. Left, representative images. Right, statistical analysis. Average fold change was labeled on the graph. Scale bars: 100 μm. n = 3, biological replicates (f) Transwell assay in WT and hnRNPL knockdown cells with LINC02273 overexpression. Left, representative images. Right, statistical analysis. Average fold change was labeled on the graph. Scale bars: 100 μm. n = 3, biological replicates. For (c-f), means ± s.e.m. are shown, and P values were determined by independent sample t-tests. *P < 0.05, **P < 0.01, ***P < 0.001