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Fig. 2 | Molecular Cancer

Fig. 2

From: m6A modification-mediated CBX8 induction regulates stemness and chemosensitivity of colon cancer via upregulation of LGR5

Fig. 2

CBX8 suppresses the chemosensitivity of CC Cells to L-OHP or CPT-11. a-b, Colony formation in shCBX8-transfected CC cells after treatment with L-OHP or CPT-11 a concentration of 10 μmol/L. c-d, Colony formation in CBX8 overexpression plasmid-transfected CC cells after treatment with L-OHP or CPT-11 a concentration of 10 μmol/L. e-f, Flow cytometry was used to assess the apoptotic rate of CBX8-silenced DLD-1 cells and CBX8-overexpressed SW480 cells when treated with L-OHP or CPT-11 a concentration of 10 μmol/L. g, Representative images of xenograft tumors in nude mice after different treatments. Vec or shCBX8 were injected intratumorally once per week for 4 weeks. L-OHP or CPT-11 were injected intraperitoneally twice per week for 4 weeks. The combination of shCBX8 with L-OHP or CPT-11 had stronger inhibitory effects on tumor growth. h, The tumor growth curves of each group of mice are summarized. i, The levels of CBX8 and caspase 3 expression were assessed by IHC in different groups. Data are shown as the mean ± SD of three replicates (*, P < 0.05; **, P < 0.01)

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