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Fig. 1 | Molecular Cancer

Fig. 1

From: m6A mRNA methylation regulates CTNNB1 to promote the proliferation of hepatoblastoma

Fig. 1

Frequent up-regulation of m6A methylation in HB and m6A factors affected HB cell proliferation and apoptosis. a Elevation of m6A mRNA methylation level in HB tumor and normal samples determined by m6A enzyme-linked immunosorbent assay (paired samples t-test). b mRNA expression of m6A regulators in 16 pairs of HB determined by RT-qPCR (paired samples t-test). c Protein expression of m6A regulators validated in 8 pairs of HB and nontumor samples by western blotting. d HepG2 cells were transfected with siMETTL3, siWTAP, siFTO, siYTHDF2 and the knockdown effect was verified at mRNA and protein levels (one-way analysis of variance, Dunnett’s test). e HepG2 cells were transfected with siMETTL3, siWTAP, siFTO, siYTHDF2 followed by CCK8 assays. (one-way analysis of variance, Dunnett’s test). f Knockdown of METTL3, WTAP, FTO, YTHDF2 in HepG2 cells subjected to colony formation assays, histogram of colony formation assays from 3 independent experiments (one-way analysis of variance, Dunnett’s test). g Flow cytometry assays used to evaluate the apoptosis in HepG2 cells transfected with siMETTL3, siWTAP, siFTO, siYTHDF2, histogram of flow cytometry assays from 3 independent experiments (one-way analysis of variance, Dunnett’s test). Abbreviations: N, normal; T, tumor. *p-value< 0.05, **p-value< 0.01, ***p-value< 0.001, ****p-value< 0.0001

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