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Fig. 6 | Molecular Cancer

Fig. 6

From: Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomas

Fig. 6

circ-MAPK4 regulates p38/MAPK4 signaling pathway by targeting miR-125a-3p. (a) Expression of circ-MAPK4 and miR-125a-3p are inversely correlated in glioma tissues (R2 = -0.2394, P = 0.0061). (b) qPCR was performed to test silence effect of the miR-125a-3p inhibitor. (c) Western blot assays evaluated phosphorylation and total protein levels of p38/MAPK in circ-MAPK4 silenced U373 cells which were treated either with or without miR-125a-3p inhibitor. (d, e) Apoptosis and western blot assays revealed levels of cellular apoptosis and apoptosis effectors after inhibiting miR-125a-3p in circ-MAPK4 silenced U373 cells. Intensity of bands were scanned and measured by Image J software, and summarized as bar graph. All experiments were performed at least three times. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001

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