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Fig. 2 | Molecular Cancer

Fig. 2

From: Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response

Fig. 2

LUCAT1 inhibits DNA damage and apoptosis of CRC cells. a and b CCK8 and colony formation assays of HCT-116 and LoVo following NC or LUCAT1 knockdown. n = 3 independent experiments, two-tailed Student’s t-test. c GSEA analyses of RNA-seq data from HCT-116 cells transfected with siNC or LUCAT1 siRNAs. n = 180, pearson correlation. d Cell cycle analyses of HCT-116 and LoVo cells transfected with siNC or LUCAT1 siRNAs. n = 3 independent experiments, two-tailed Student’s t-test. e Protein expression levels of a DNA damage marker, p-H2AX, following NC or LUCAT1 knockdown as measured by western blotting. Integrated Density Value (IDV) was obtained by ImageJ and normalized by the first lane. f Confocal microscopic images of p-H2AX in HCT-116 and LoVo cells after NC or LUCAT1 knockdown. g Caspase 3/7 activities of HCT-116 and LoVo cells following NC or LUCAT1 knockdown without or with CoCl2 treatment. n = 3 independent experiments, two-tailed Student’s t-test. h Tumor volume and tumor weight of NC or LUCAT1 overexpressing HCT-116 cells in xenograft mouse model. n = 8 tumors, two-tailed Student’s t-test. * p < 0.05, ** p < 0.01, and *** p < 0.001

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