Fig. 3
From: Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response
LUCAT1 interacts with PTBP1 in CRC cells. a Silver staining and mass spectrometry analyses following RNA pull down of LUCAT1 sense or LUCAT1 antisense in HCT-116 and RKO cells. b and c Western blotting validation of biotin-labelled RNA pull down using antisense or sense probes and GST pull down using empty vector or LUCAT1-MS2. d RIP assays were performed in HCT-116 and RKO cells. n = 3 independent experiments, two-tailed Student’s t-test. e Western blot of PTBP1 and STAU1 in biotinylated antisense, full length or truncated LUCAT1 pull down. f Colocalization of LUCAT1 and PTBP1 in CoCl2-treated HCT-116 and RKO cells. g Deletion mapping to identify the LUCAT1 binding domain in PTBP1 by RIP-qPCR using full length or truncated PTBP1 protein. * p < 0.05, ** p < 0.01, and *** p < 0.001