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Fig. 4 | Molecular Cancer

Fig. 4

From: Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response

Fig. 4

LUCAT1 modulates alternative splicing via interaction with PTBP1. a GSEA analyses of LUCAT1 knock down HCT-116 cells and PTBP1 knock down HCT-116 cells. Dots in colors are statistically significant. Dots in red are genesets involving cell growth, cell cycle and G2/M checkpoint. b Venn diagram of splicing events regulated by LUCAT1 and PTBP1. c Gene annotation and analysis of common genes alternatively spliced by LUCAT1 and PTBP1. d Exon coverage viewed in IGV. Arrows in orange indicates the corresponding exons. e Alternative splicing events in each target gene were determined by qPCR and normalized to siNC-transfected CRC cells. n = 3 independent experiments, two-tailed Student’s t-test. f Alternative splicing of target genes in CRC cells transfected with siNC, LUCAT1 siRNAs or PTBP1 siRNAs was validated by RT-PCR. g and h PTBP1-RIP assays were performed following LUCAT1 knockdown or overexpression. n = 3 independent experiments, two-tailed Student’s t-test. i Correlation between target gene alternative splicing and LUCAT1 expression in CRC tissues. n = 46 in CRC cohort, pearson correlation. * p < 0.05, ** p < 0.01, and *** p < 0.001

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