Fig. 1

IFNα-induced lncMX1–215 expression is dependent on p-stat1 in HNSCC cells. a lncRNA sequencing was performed in Cal27 and HN30 cells after treatment with 200 ng/ml IFNα for 24 h. b, c The relative ENST00000486275 expression level under IFNα treatment at the indicated dose and time was detected using qRT-PCR. d Agarose gel electrophoresis for 3′ and 5′ RACE analysis of lncMX1–215 was shown. e The distribution of lncMX1–215 was analyzed via FISH in HN30 cells. 18S RNA and U6 indicate the cytoplasm and nucleus, respectively. f The subcellular distribution of lncMX1–215 was analyzed via PCR in HNSCC cell lines. g lncMX1–215 expression was detected in HNSCC cell lines and normal oral keratinocytes. h The binding sites and stat1 motif in the promoter region of lncMX1–215 is shown. ChIP assays were performed after treatment with 200 ng/ml IFNα for 24 h. i Transcriptional activity was detected using dual-luciferase reporter assays with lncMX1–215 promoter-luciferase truncations in 293 T cells. j After transfection of 293 T cells with the indicated promoter truncations for 24 h, the cells were treated with the indicated IFNα stimulation for another 24 h. Then, the transcriptional activity was detected using a dual-luciferase reporter assay. Scale bars, 100 μm; *: P < 0.05; **: P < 0.01