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Fig. 3 | Molecular Cancer

Fig. 3

From: A novel IFNα-induced long noncoding RNA negatively regulates immunosuppression by interrupting H3K27 acetylation in head and neck squamous cell carcinoma

Fig. 3

Gain of H3K27 acetylation activates PD-L1 and galectin-9 expression. a Layered H3K27 acetylation enrichment in the upstream region of PD-L1 and LGALS9, as shown in the UCSC database. b PD-L1 and acetylation of histone 3 were detected and quantified after treatment with 200 ng/ml IFNα or 5 μM MS-275 for 24 h. # indicated the deference between combined group and each alone. c PD-L1, H3K27ac, H3K18ac and H3K9ac were detected and quantified after the indicated MS-275 treatment for 24 h. d PD-L1 and H3K27ac were detected and quantified after 5 μM MS-275 treatment for the indicated time. e After treatment of HN4 and Cal27 cells with 15 μM SAHA or 5 μM MS-275 for 24 h, ChIP assays were performed using anti-H3K27ac antibody. f The promoter activity of PD-L1 and LGALS9 was measured after transfection with lncMX1–215 for 24 h and then SAHA or MS-275 treatment for 24 h in 293 T cells. g, h The relative mRNA of CD274 and LGALS9 were detected after transfection with lncMX1–215 and then 15 μM SAHA or 5 μM MS-275 treatment for 24 h in HN4 and Cal27 cells. *: P < 0.05; **: P < 0.01

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Molecular Cancer

ISSN: 1476-4598