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Fig. 6 | Molecular Cancer

Fig. 6

From: A novel IFNα-induced long noncoding RNA negatively regulates immunosuppression by interrupting H3K27 acetylation in head and neck squamous cell carcinoma

Fig. 6

LncMX1–215 inhibits tumor proliferation capacity in vitro and in vivo. a, b The viability of HN4 and Cal27 cells after transfection with lncMX1–215 or ASO was determined using CCK8 assays. c, d Colony formation assays were performed with HN4 and Cal27 cells after transfection with lncMX1–215 or ASO. e, f EdU assays were performed after transfection with lncMX1–215 or ASO. g PARP and cleaved caspase 3 were detected via western blotting after transfection with lncMX1–215 for 48 h. h Cell apoptosis was analyzed via flow cytometry using an Annexin V/7-AAD kit after transfection for 48 h. i Cell cycle was analyzed using flow cytometry after transfection for 48 h. j Tumor growth curves for mice injected with cells treated with lncMX1–215 lentivirus or vector were analyzed. k Tumors derived from the xenograft model were resected and are shown for each group. l The weight of tumors resected from mice in the ectopic expression and vector groups was measured and analyzed. m The percentage of TUNEL-positive cells in each group was compared. n The relative Ki-67 staining score in each group was analyzed. *: P < 0.05; **: P < 0.01

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Molecular Cancer

ISSN: 1476-4598