Fig. 2

Overexpression of ALKBH5 results in sensitization of cancer cells to chemotherapy. a Colony formation of MIA Paca-2 cells transfected with control (upper panel) or ALKBH5 (bottom panel) vector with gemcitabine treatment of different doses (0, 10, 20 nM). b Dose response curves of gemcitabine in BxPC-3 cells expressing the indicated vectors. c Correlation of ALKBH5 expression and IC₅₀ of gemcitabine. d Quantification of Colony formation. e Immunoblotting to measure ALKBH5 protein levels in AsPC-1 cells transfected with shCtr and/or shALKBH5 and/or ALKBH5 vectors. WT, wildtype; KD, ALKBH5 knockdown; OV, ALKBH5 overexpression. f Quantification of Colony formation assay with cells described in (e). g Kaplan-Meier analysis indicating overall survival of xenograft mice injected with gemcitabine-treated AsPC-1 cells bearing ALKBH5 injected with gemcitabine-treated AsPC-1 cells bearing ALKBH5 shRNA (red) or control (green). h Tumor growth rate of xenograft mice described above. i Representative images of tumor volumes from xenograft mice described above. Representative images (j) and its quantification (k) of immunohistochemistry (IHC) staining for Ki67 in tumor sections from xenograft mice described above. Scale bar = 200 μm in j. The data are shown as the means ± S.D. **P <0.01