Fig. 3

Knockdown of ALKBH5 significantly increased PDAC cell proliferation, colony formation, and migration. a Immunoblotting (upper panel) and qRT-PCR (bottom panel) to measure ALKBH5 expression in AsPC-1 (left) and PANC-1 (right) cells transfected with shCtr and/or sh ALKBH5. b MTT, (c) Colony formation, (d) Quantification of (c), and (e) Migration (upper) and invasion (lower panel) assay of cells described in (a). f Representative ventral view images and its quantification (g) of bioluminescence from xenograft mice implanted with AsPC-1 cells described in (a). h Representative images (left) and its quantification (right) of immunohistochemistry (IHC) staining for Ki67 in tumor sections from xenograft mice described above. i Representative ventral view images and its quantification (j) of bioluminescence for liver metastasis from xenograft mice described above. k Table summarizing the result of liver metastasis. Scale bars, 50 μm (e) and 100 μm (h). The data are shown as the means ± S.D. **P <0.01