Fig. 4

Overexpression of ALKBH5 inhibited PDAC cells proliferation, colony formation, cell migration and tumor growth in a nude mice model. a Immunoblotting (left panel) and qRT-PCR (right panel) to measure ALKBH5 expression in BxPC-3 (upper) and MIA Paca-2 (bottom) cells transfected with control and/or ALKBH5 vector. b MTT, (c) Colony formation and its quantification, and (d) Migration (upper) and invasion (lower panel) assay of cells described in (A). (E) Representative ventral view images and its quantification (f) of bioluminescence from xenograft mice implanted with BxPC-3 cells described in (a). g Representative images (upper) and its quantification (bottom) of IHC staining for Ki67 in tumor sections from xenograft mice described above. h Representative ventral view images and its quantification of bioluminescence for liver metastasis from xenograft mice described above. Scale bars, 50 μm (d) and 100 μm (g). The data are shown as the means ± S.D. **P <0.01