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Fig. 6 | Molecular Cancer

Fig. 6

From: m6A demethylase ALKBH5 inhibits pancreatic cancer tumorigenesis by decreasing WIF-1 RNA methylation and mediating Wnt signaling

Fig. 6

WIF-1 as a downstream target of ALKBH5-mediated m6A modification. a qRT-PCR to measure WIF-1 expression in AsPC-1 (left) and PANC-1 (middle) cells transfected with shCtr and/or sh ALKBH5, and MIA Paca-2 (right) cells transfected with control and/or ALKBH5 vector. b Methylated RNA immunoprecipitation (MeRIP)-qPCR analysis of m6A levels of WIF-1 pre-mRNA in transformed cells described above. c Luciferase reporter assays showing the impact of ALKBH5 overexpression on WIF-1 promoters in BxPC-3 cells. d qRT-PCR to measure WIF-1 expression in transformed AsPC-1 and PANC-1 cells described above with or without 3-deazaadenosine (DAA), the global methylation inhibitor. The data are shown as the means ± S.D. **P <0.01; ## <0.01

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