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Fig. 1 | Molecular Cancer

Fig. 1

From: Circular RNA CRIM1 functions as a ceRNA to promote nasopharyngeal carcinoma metastasis and docetaxel chemoresistance through upregulating FOXQ1

Fig. 1

CircCRIM1 is upregulated in NPC patients with distant metastasis and promotes NPC cell metastasis and EMT in vitro. a Volcano plots of differential circRNAs between high metastasis S18 cells and low metastasis S26 cells. b Genomic loci of the circCRIM1 gene. The red and green arrows represent divergent primers. The backsplicing junction was validated by Sanger sequencing. c Quantitative real-time PCR (qRT-PCR) analysis of GAPDH, CRIM1 mRNA and circCRIM1 expression after RNase R treatment. d circCRIM1 expression in cDNA and genomic DNA (gDNA). GAPDH was used as a negative control. e Relative expression of circCRIM1 in S18, HONE1 and S26 cells. f Relative expression of circCRIM1 in NPC tissue without (n = 157) or with (n = 61) distant metastasis. g-h Representative and quantified results of the Transwell migration and invasion assays in NPC cells transfected with circCRIM1 siRNA or scrambled control (siSCR, g) or with circCRIM1 overexpression plasmid or vector (h). Scale bar, 100 μm. i Immunofluorescence images of E-cadherin and Vimentin expression in NPC cells with or without circCRIM1 downregulation. Scale bar, 10 μm. j Western blotting analysis of E-cadherin, N-cadherin and Vimentin in NPC cells with or without circCRIM1 depletion. The data are presented as the mean ± S.D. All in vitro data are representative of three independent experiments. Student’s t-test; *P < 0.05

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