Fig. 1

Characterization of lncRNA P14AS in cancer cells. a Illustration of the location of CDKN2A RNA capture probes (30 k) and lncRNA P14AS transcribed from the antisense strand of the sequence around exon 1β of the CDKN2A gene (chr9: 21,989,178-21,994,898 in the human genome GRCh37/hg19). Sequences for the three exons are highlighted with different colors: exon 1 in gray, exon 2 in blue, and exon 3 in green. AT (AU in RNA) sites are marked with red letters. b The expression status of P14AS in various human cell lines by Northern blotting. An 80-nt P14AS-specific probe was labeled with ³²P for Northern blot analysis. The 18S rRNA and 28S rRNA were used as RNA controls. c cDNA synthesized from total RNA with or without RNase R digestion was used as the PCR template. d Cytoplasm P14AS (red) was visualized by RNA-FISH in HEK293T and SW480 cells, but not in A459 cells without the CDKN2A/B locus. P14AS-specific probes were labeled with Cy3 dye. The 18S rRNA and U6 RNA were used as the cytoplasm and nucleus RNA controls, respectively. Scale bar, 5 μm