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Fig. 2 | Molecular Cancer

Fig. 2

From: Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells

Fig. 2

P14AS interacts with AUF1 through the AU-rich element in exon 1. a Biotin-labeled P14AS pull-down complexes from HEK293T cell lysate, following mass spectrometry; (b) Western blot analyses; (c) Interaction of AUF1 protein with P14AS in RIP-PCR; (d) Interaction of four purified AUF1 protein isoforms with biotin-labeled P14AS probe in the RNA-electrophoretic mobility shift assay (RNA-EMSA). The interaction of Band #2-AUF1 P42 isoform with biotin-labeled P14AS probe was almost completely inhibited by the unlabeled probe (×10). However, the interaction of Band #1-AUF1 P42 and P45 isoforms with the P14AS was not affected by the unlabeled probe

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