Fig. 4
From: Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells
P14AS promoted cancer cell proliferation. aP14AS overexpression promoted cell proliferation in the CCK-8 assays and IncuCyte long-term dynamic observation. b Wound width curve for HCT116 and SW480 cells with P14AS overexpression in the long-term observation. c Effect of P14AS knockout (KO) by CRISPR/Ccas9 and P14AS re-expression in the proliferation of HCT116 and HEK293T cells. d Effect of shRNA-knockdown of AUF1 and/or transient P14AS overexpression on the proliferation of HCT116 cells (Right chart). The levels of AUF1 and ANRIL expression in HCT116 cells with stable shAFU1 transfection for four weeks was tested with qRT-PCR (Left and Right charts, respectively) and Western blotting (top chart) before these cells were transiently transfected with the P14AS expression vector. Student’s t-test: *, P < 0.05; **, P < 0.01