Fig. 6
From: Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells
Effects of the knockout of the P14AS promoter on the P14AS and ANRIL transcription and the proliferation of HEK293T cells. (a) Remaining sequences of the P14AS promoter with a 653 bp deletion (chr9:21,986,875-21,987,527; hg19) by CRISPR/Cas9 in two HEK293T subclones (KO-3 and KO-4). Locations of the wildtype P14AS promoter, two guide RNAs, and the 653 bp knockout (KO) fragment are also illustrated; (b and c) The levels of P14AS and ANRIL expression in wildtype and KO-3 or KO-4 cells by qRT-PCR. (d and e) Effect of the promoter deletion on KO-3 and KO-4 cell proliferation in the IncuCyte long-term dynamic observation. Pooled P14AS promoter-KO-negative clones were used as a wild-type (WT) control. Error bars, S.D. **, P < 0.01