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Fig. 3 | Molecular Cancer

Fig. 3

From: Autophagy-associated circRNA circCDYL augments autophagy and promotes breast cancer progression

Fig. 3

circCDYL promotes proliferation of breast cancer cells via autophagy. a qRT-PCR analysis of circCDYL in MDA-MB-231 cells after Baf A1 (Bafilomycin A1) treatment. b LC3 expression in MDA-MB-231 after circCDYL silencing via siRNA or over-expressing circCDYL by over-expressing plasmid, as detected by Western Blot. LC3-II/I, the gray value ratio of LC3-II and LC3-I, normalized to NC group. c Autophagosomes in mCherry-GFP-LC3 labeled MDA-MB-231 after silencing circCDYL via siRNA or over-expressing circCDYL by over-expressing plasmid, as detected by confocal microscopy. Scale bar = 50 μm. d, e The proliferation of MDA-MB-231 and MCF-7 cells after silencing circCDYL viasiRNA or over-expressing circCDYL via over-expressing plasmid, as detected by cell viability assay (d) and plate colony formation (e). f, g Proliferation of MDA-MB-231 and MCF-7, as detected by plate colony formation (f) and cell viability assay (g) after transfection with circCDYL over-expressing plasmid or co-treatment with Baf A1. siNC: negative control siRNA. All experiments above were repeated at least 5 times. *P < 0.05, **P < 0.01, ***P < 0.005. Error bars indicate S.E.M

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