Fig. 3

PLACT1 overexpression promotes a significant effect on tumorigenesis and metastatic potential in vivo. a-b Gross appearance of xenograft tumors after subcutaneous injections with sh-NC and sh-PLACT1#1 group (n = 10). c-d Tumor volumes (c) and weights (d) were measured in the indicated groups (n = 10). e-f Representative images (e) of IHC for Ki-67. Histogram analysis (f) revealed that PLACT1 was associated with Ki-67 expression (n = 10). Scale bars: 50 μm. g Representative images of lung colonization after injection of PANC-1 cells into the tail veins of mice (n = 10). Scale bars: 50 μm. h-i Histogram analysis for luminescence (h) and the number (i) of metastatic foci representing lung metastasis (n = 10). j The ratio of lung metastasis was calculated for indicated group (n = 10). Statistical significance was assessed using two-tailed t-tests and ANOVA followed by Dunnett’s tests for multiple comparison. Error bars represent standard deviations of three independent experiments. *p < 0.05 and **p < 0.01