Fig. 6

PLACT1 activates the NF-κB signaling pathway. a-b Genes involved in the NF-κB signaling pathway were detected by qRT-PCR in PLACT1 knockdown (a) or overexpression (b) cells. c-d Western blotting analysis showed the changed protein levels involved in the NF-κB signaling pathway after knockdown (c) or overexpression (d) of PLACT1 in PANC-1 cells. e Representative images of immunofluorescence analysis showed that PLACT1 induced P65 translocation in PANC-1. f-g qRT-PCR (f) and western blotting (g) analysis indicated that PLACT1 downregulated the mRNA and protein levels of IκBα in PANC-1 after treatment with BAY 11–7085. h PLACT1 was negatively correlated with IκBα in 166-case of PDAC tissues. i, Western blotting analysis showed that PLACT1-mediated P65 translocation can be reversed by JSH-23. j-k Representative images and histogram analysis of EdU (j) and Transwell (k) assays showed that JSH-23 reversed the effects of PLACT1-overexpressing PANC-1 cells. Scale bars: 100 μm. p-values were calculated by using two-tailed t-tests and ANOVA followed by Dunnett’s tests for multiple comparison. The error bars represent standard deviations of three independent experiments. *p < 0.05 and **p < 0.01