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Fig. 4 | Molecular Cancer

Fig. 4

From: Cytosine methylation of mature microRNAs inhibits their functions and is associated with poor prognosis in glioblastoma multiforme

Fig. 4

Cytosine-methylation of miRNA-181a-5p abolishes the formation of miRNA-181a-5p-3’UTR/BIM duplex. a The graph illustrates the relative presence of 3’UTR/BIM on biotinylated miRNA according to the previous method. b The graph illustrates the relative presence of miRNA-181a-5p on 3’UTR/BIM on biotinylated miRNA according to the previous method. c The graph illustrates the miRNA-150-5p and miRNA-181a-5p enrichments on GW182 and IgG (negative control). Experiments were performed using the RiboCluster Profiler kit (CliniScience, France) according to manufacturer’s instructions. d The graph illustrates the 3’UTR/BIM and 3’UTR/EP300 enrichments on GW182 and IgG (negative control). Experiments were performed using the RiboCluster Profiler kit (CliniScience, France) according to the manufacturer’s instructions

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Molecular Cancer

ISSN: 1476-4598