Fig. 2
From: Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance
MUC5AC-expressing colon cancer cells exhibit a higher percentage of stem cells or side population. a Schematic depicting the location of gRNAs (at exon 2) of CRISPR/Cas9 used for knocking out the MUC5AC gene. b and c Immunoblot showing deletion of MUC5AC in clones of CRC cell lines (HCT-8 and LS174T). d and e Side or stem cell population (SP) was isolated in the presence of an ATP-binding cassette inhibitor (verapamil). A higher SP percentage was observed in HCT-8 and LS174T parental cells as compared to MUC5AC knockout tumor cells (*p < 0.05 and **p < 0.01)