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Fig. 3 | Molecular Cancer

Fig. 3

From: Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance

Fig. 3

MUC5AC expression results in the maintenance of a stem-like phenotype in colon cancer cells. a Side or stem cell population (SP) cells were isolated based on Hoechst exclusion staining via FACS and expanded by culturing the cells in the specific stem cell growth media. Western blot analysis showed an increase in MUC5AC and CD44 levels in parental SP cells. b and c Morphology analyses of SP and non-side population (NSP) of parental and KO clones isolated from HCT-8 and LS174T cells. d and e Formation of colon spheres/tumor spheres in parental and KO clones (Clone-1 and -2) from HCT-8 and LS174T after 10 days. A significant decrease in spheroid formation was observed in KO clones as compared to parental cells (*p < 0.05)

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