Fig. 7
From: Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance
Presence of MUC5AC mediates resistance to 5-FU. a 5-FU treatment at 1 and 5 μM for 48 h increased expression of MUC5AC in HCT-8 and LS174T cell lines. b Similarly, in the presence of oxaliplatin both cell lines showed upregulation of MUC5AC expression. c DNA content in different phases of the cell cycle was assessed by propidium iodide staining of parental and MUC5AC knockout clones (Clone-1 and -2) followed by FACS analysis. Cell cycle analysis showed that in the absence of MUC5AC, cells were arrested at G2/M phase. d The presence of MUC5AC facilitates 5-FU resistance via the β-catenin/p53/p21 axis and via upregulation of β-catenin target genes Lgr5 and CD44. e Schematic diagram showing MUC5AC mediates CRC cell survival, migration, and invasion and confers 5-FU resistance via Src and β-catenin signaling, respectively