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Fig. 3 | Molecular Cancer

Fig. 3

From: RNA demethylase ALKBH5 prevents pancreatic cancer progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent manner

Fig. 3

ALKBH5 attenuation of proliferation, cell cycle, migration and invasion of PC cells. ALKBH5 overexpression significantly decreased the proliferation rate a, e and weakened the colony-formation ability c of BxPC-3 cells, whereas ALKBH5 knockdown promoted SW1990 cell growth, as measured by the CCK-8, EdU b, f and colony formation d assays. Flow cytometry combined with western blotting revealed that ALKBH5 overexpression (Lv-ALKBH5 group) induced remarkable increase in the percentage of BxPC-3 cells in the G2/M phase, and lead to Cyclin B1 decrease and p-CDK1 increase g. Significantly decreased cell number in the G2/M phase with Cyclin B1 increase and p-CDK1 decrease was observed in condition of ALKBH5 knockdown (shALKBH5 group) h. Both wound healing and transwell assays showed the suppressed migration i and invasion k of PC cells, respectively, following ALKBH5 overexpression. In contrast, ALKBH5 knockdown promoted both migration (wound closure) j and invasion l of PC cells. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598