Fig. 3

CircMRPS35 Inhibits Gastric Cancer Cells Proliferation and Metastasis in vitro. a qRT-PCR assay for circMRPS35 expression in different human gastric cancer cell lines and GES-1. b qRT-PCR analysis of circMRPS35 and MRPS35 mRNA after the transfection of overexpression plasmids into SGC7901 and MKN28 cells for 24 h. (c) CCK-8 analysis of the above cells in b on the indicated days. d Flow cytometry of the cell cycle of the above cells in b. e Cell invasion assay after overexpression of circMRPS35. Six hours after the transfection of overexpression plasmid, SGC7901 and MKN28 cells were resuspended and seeded in transwells for another 48 h, and the number of invasion cells was calculated. f Schematic illustration of two siRNAs targeting the back-splice junction of circMRPS35 (Si1 and Si2). g qRT-PCR assay of interfering efficacy after transfection of the siRNAs into MGC803 cells for 24 h. h-j CCK-8 h, flow cytometry i and cell invasion assay j of the above cells in g. *P < 0.05, **P < 0.01, ***P < 0.001