Fig. 7
ALKBH5 inhibits tumor growth and metastasis by reducing the expression and activity of YAP in YTHDF1/2 and miR-107-dependent manner in vivo. (a) The expressions of ALKBH5 and YTHDF1 were analyzed by RT-PCR and qPCR assays. (b, c) Xenografted A549 cell tumors with stable expression of indicated genes in mice (b) and the dimensions measured at regular intervals (c). (d) The overall survival (OS) curves of the mice from transfected of A549 cells with stable expression of indicated genes. (e, f) The protein (e) and mRNA (f) levels of Ki67, YAP, CTGF, E-cadherin and Vimentin were detected in xenografted A549 cell tumors with stable expression of indicated genes determined by immunohistochemical staining (n = 5) and qPCR assays. (g) Representative H&E stained microscopic images of the mice metastatic lung tumors. (h) The RNA levels of ALKBH5 and miR-107 were analyzed by RT-PCR and qPCR in the A549 cells with stable expression of indicated genes. (i, j) Xenografted A549 cell tumors with stable expression of indicated genes in mice (i) and the dimensions measured at regular intervals (j). (k) The OS curves of the mice with indicated A549 cells. (l, m) The mRNA (l) and protein (m) levels of indicated genes were detected in relevant xenografted A549 cell tumors determined by RT-PCR and western blot assays. (n) Representative H&E stained microscopic images of the mice metastatic lung tumors. (o, p) Xenografted A549 cell tumors with stable expression of indicated genes (o) and the dimensions measured at regular intervals in mice with treatment of vehicle or cycloleucine (CL) (p). (q) The protein levels of indicated genes were detected in relevant xenografted A549 cell tumors determined by IHC assay. (r) Representative H&E stained microscopic images of the mice metastatic lung tumors. Results were presented as mean ± SD of three independent experiments. **P < 0.01 indicates a significant difference between the indicated groups