Fig. 4

CircMUC16-miR-199a-5p-Beclin1 axis regulated autophagy. a The expression of miRNAs of SKOV3 cells was determined by qPCR. b The expression of miRNAs of A2780 cells was determined by qPCR. c The location of miR-199a and circMUC16 in SKOV3 cells was detected using FISH assay. d RNA pulled down assay was performed. Then, the expression of circMUC16 was detected by qPCR. e RNA pulled down assay was performed. Then, the expression of miR-199a was detected by qPCR. f The expression of Beclin1 and ATG14 of SKOV3 cells was determined using western blot. f SKOV3 cells were transfected with LV2-NC, LV2–1, LV2–2, LV2–1 + miR-199a inhibitors, miR-199a mimics, miR-199a mimic inhibitors. Then, the expression of Beclin1 and ATG14 were detected using western blot. g SKOV3 cells were co-transfected with miR-199a mimic or control RNA (NC) using luciferase reporter plasmids containing either wild-type (pMIR-CircMUC16) or mutant (pMIR-CircMUC16m). Luciferase expression was measured. Error bars represent the standard error. The symbols * and ** indicate p < 0.05 and 0.01, respectively