Fig. 5
From: CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a
CircMUC16 regulated RUNX1 via sponging miR-199a-5p. a The expression of RUNX1 was determined by western blot. b SKOV3 cells were co-transfected with miR-199a mimic or control RNA (NC) using luciferase reporter plasmids containing either wild-type (pMIR-RUNX1 3’UTR) or mutant (pMIR-RUNX1 3’UTRm). Luciferase expression was measured. c A2780 cells were transfected with LV6, LV6-NC, LV6 + RUNX1 siRNA, or LV6 + Beclin1 siRNA. After 48 h, the expression of LC3-II was determined by western blot. d, e A2780 cells were transfected with LV6, LV6-NC, LV6 + RUNX1 siRNA, or LV6 + Beclin1 siRNA. Then, mRFP-GFP-LC3 distributions were determined by confocal microscopy. Error bars represent the standard error. The symbols * and ** indicate p < 0.05 and 0.01, respectively. Scale bar: 5 μm