Fig. 7
From: CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a
CircMUC16 directly bound to ATG13 protein. a The circMUC16 RNA pulldown was performed. Then, silver stains was obtained. b The circMUC16 specific binding protein gels was identified by RNA pulldown. c The expression of ATG13 protein was determined by western blot. d The potential interaction sites between circMUC16 and ATG13 protein were predicted using catRAPID. e Segments of ATG13 DNA were cloned into a PCMV5 plasmid. SKOV3 cells were transfected with PCMV5 vector, PCMV5-ATG13, PCMV5-△51–102, PCMV5-△200–251 or PCMV5-△475–526. After 48 h, the expression of circMUC16 was determined. Error bars represent the standard error. The symbols * and ** indicate p < 0.05 and 0.01, respectively