Fig. 3
From: CAF secreted miR-522 suppresses ferroptosis and promotes acquired chemo-resistance in gastric cancer
CAFs secrete exo-miR-522 to suppress ferroptosis of GC cells. a. TEM images of exosomes isolated from primary NFs, TCs and CAFs (scale bar, 100 nm). b. WB analysis of exosomal markers in the exosomes isolated from GC cell lines and CAFs. c. Quantification of miR-522 in both cells and exosomes (n = 3). d. Confocal microscopy image of the internalization of fluorescently labeled CAF exosomes in SGC7901 cells and MKN45 cells. Scale bars, 50 μm. e. Effects of CAF-secreted miR-522 on the expression of ALOX15 in GC cells (n = 3). f. Quantitative analysis of (e) (n = 3). g. Relative levels of ALOX15 mRNAs in GC cells treated as described above (n = 3). h-k. Exo-miR-522 derived from CAFs suppresses erastin-induced ferroptosis in GC cells. CAF exo-miR-522 inhibits lipid-ROS accumulation (h), decreases erastin-induced cell death (i) and reduces abnormal increase of MMP (j, k). ** indicates p < 0.01 and *** indicates p < 0.001